magnetic bead protein rna complexes (New England Biolabs)

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New England Biolabs magnetic bead protein rna complexes
Magnetic Bead Protein Rna Complexes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+protein+complexes/Protein+A+Magnetic+Beads/pmc12777463-451-30-79
Average 96 stars, based on 676 article reviews

magnetic bead protein rna complexes - by Bioz Stars, 2026-09

96/100 stars

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Isolation:

Article Title: The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roles
Article Snippet: .. RNA-protein complexes were isolated with oligo[dT)] 25 magnetic beads (New England Biolabs, S1419S). ..

Article Title: The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roles
Article Snippet: .. RNA- protein complexes were isolated with oligo[dT]25 magnetic beads (New England Biolabs, S1419S). ..

Article Title: The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roles
Article Snippet: .. RNA-protein complexes were isolated with oligo[dT] 25 magnetic beads (New England Biolabs, S1419S). ..

Magnetic Beads:

Article Title: Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing
Article Snippet: .. RNA-protein complexes were captured on oligo(dT)25 magnetic beads (NE Biolabs) for 1 hour at 4°C with gentle rotation. ..

Article Title: The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roles
Article Snippet: .. RNA- protein complexes were isolated with oligo[dT]25 magnetic beads (New England Biolabs, S1419S). ..

Article Title: OTC intron 4 variations mediate pathogenic splicing patterns caused by the c.386G>A mutation in humans and spf ash mice, and govern susceptibility to RNA-based therapies.
Article Snippet: To affinity purify RNA-binding proteins, 100 pmol of 3′-biotin-coupled RNA oligonucleotides (Integrated DNA Technologies, Coralville, IA, USA) were incubated with 24 μg of HeLa nuclear extract (Ipracell, Mons, Belgium) and protease inhibitor cocktail (ThermoFisher Scientific, Waltham, MA, USA) in TENT buffer (10 mM Tris, 1 mM EDTA, 250 mM NaCl, 0.5% Triton X-100, pH 7.5) for 30 min at room temperature (20–25 °C). .. RNA– protein complexes were bound with 50 μg of streptavidincoated magnetic beads (New England Biolabs, Ipswich, MA, USA) and washed with TENT buffer. .. Proteins were eluted with SDS sample buffer and analyzed by Western blotting with monoclonal mouse anti-TIA1 (0TI1D7, ThermoFisher Scientific, Waltham, MA, USA), polyoclonal mouse anti-Sam68 (PA5-62364, ThermoFisher Scientific, Waltham, MA, USA), monoclonal anti-GAPDH (ZG003, ThermoFisher Scientific, Waltham, MA, USA) and then with polyclonal goat anti-mouse HRP-conjugated (P0447, Dako, Jena, DE) antibodies.

Article Title: The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roles
Article Snippet: .. RNA-protein complexes were isolated with oligo[dT] 25 magnetic beads (New England Biolabs, S1419S). ..

Article Title: OTC intron 4 variations mediate pathogenic splicing patterns caused by the c.386G>A mutation in humans and spf ash mice, and govern susceptibility to RNA-based therapies
Article Snippet: To affinity purify RNA-binding proteins, 100 pmol of 3′-biotin-coupled RNA oligonucleotides (Integrated DNA Technologies, Coralville, IA, USA) were incubated with 24 μg of HeLa nuclear extract (Ipracell, Mons, Belgium) and protease inhibitor cocktail (ThermoFisher Scientific, Waltham, MA, USA) in TENT buffer (10 mM Tris, 1 mM EDTA, 250 mM NaCl, 0.5% Triton X-100, pH 7.5) for 30 min at room temperature (20–25 °C). .. RNA–protein complexes were bound with 50 μg of streptavidin-coated magnetic beads (New England Biolabs, Ipswich, MA, USA) and washed with TENT buffer. .. Proteins were eluted with SDS sample buffer and analyzed by Western blotting with monoclonal mouse anti-TIA1 (0TI1D7, ThermoFisher Scientific, Waltham, MA, USA), polyoclonal mouse anti-Sam68 (PA5-62364, ThermoFisher Scientific, Waltham, MA, USA), monoclonal anti-GAPDH (ZG003, ThermoFisher Scientific, Waltham, MA, USA) and then with polyclonal goat anti-mouse HRP-conjugated (P0447, Dako, Jena, DE) antibodies.

Gentle:

Homogenization:

Article Title: Chronic morphine intoxication reduces binding of HuD to BDNF long 3'-UTR, while morphine withdrawal stimulates BDNF expression in the frontal cortex of male Wistar rats.
Article Snippet: Brain-derived neurotrophic factor (BDNF) mediates opiate dependence phenomenon.. In the brain of morphine dependent animals BDNF level is controlled transcriptionally, however, post-transcriptional mechanisms of BDNF regulation in this context remain unknown.. Regulation of mRNA by binding of specific proteins to the 3'-untranslated region (3’-UTR) is one of such mechanisms.

Membrane:

Article Title: System-wide analyses of the fission yeast poly(A) + RNA interactome reveal insights into organization and function of RNA–protein complexes
Article Snippet: Immunoprecipitation was performed with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) for 2 h at 4°C. .. After washing with a buffer containing 1 M NaCl, RNA–protein complexes were radiolabeled with [γ- 32 P]-ATP and T4 polynucleotide kinase (New England Biolabs), resolved on SDS-PAGE, and transferred to nitrocellulose membrane. ..

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